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Results A counter-selection system was engineered in Saccharomyces cerevisiae based on the known feedback inhibition of gamma-glutamylcysteine synthetase ( GSH1 ) gene expression, which is rate limiting for GSH synthesis: the GSH1 ORF and the counter-selectable marker GAP1 were expressed under control of the TEF1 and GSH-regulated GSH1 promoters, respectively

Upon binding, it activates a Gq-protein signaling cascade, leading to the production of inositol triphosphate (IP3) and diacylglycerol (DAG)

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But many times, you don't want the "wild-haired caffeine"