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Because your skin may already be using potent actives, layering too many can cause irritation and paradoxically more pigment

All participants provided written informed consent in accordance with the Declaration of Helsinki. Cell culture and transfection The STAD cell lines, HGC-27 and AGS (ATCC, Virginia, USA), were cultured using RPMI 1640 medium (L220KJ, basalmedia, China) and DMEM/Nutrient Mixture F-12 medium (L370KJ, basalmedia, China), respectively