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Gastrointestinal Adverse Reactions In a pool of Study 1 and 2, gastrointestinal adverse reactions occurred more frequently among patients receiving ZEPBOUND (5 mg 56%, 10 mg 56%, 15 mg 56%) than placebo (30%)

Proteins in cell lysates were resolved by SDS-PAGE electrophoresis and transferred to PVDF membrane (GE Healthcare) and subsequently incubated with blocking buffer (5% non-fat dry milk in PBS-Tween-20) for 1 h at room temperature

CD4+ T cell help confers a cytotoxic T cell effector program including coinhibitory receptor downregulation and increased tissue invasiveness