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After quantifying the concentrations of melatonin, serotonin, dopamine, glutamate, and neurotrophic factors using HPLC and ELISA, the results are analyzed using statistical software such as GraphPad Prism

In multiple comparisons of age variables, the differences between the MDD group and the control group and between the MDD group and the remission group were significant, but there was no difference between the control group and the remission group (Table 1)

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Methodology and Assumptions This analysis covers the period from January 2020 through December 2025 for both patents and scientific papers