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The samples were centrifuged, and the supernatant was subjected to a GSH assay based on a kinetic enzymatic recycling method that detects the oxidation of GSH by 5,5-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase to measure the GSH content in cells 74
Given the increasing evidence of alternative receptor-independent sources of cAMP (from intracellular sAC) (29, 30), we hypothesized that GLP-1(2836) mediates its GLP-1Rindependent effects through sAC
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