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The primary inoculum (1 mL) was transferred into 140 mL of fresh LB medium in a 500 mL flask and cultured for 8 h to obtain the secondary inoculum, which was inoculated directly into the bioreactor
Vitamin B12 is typically found in food groups such as meats, such as poultry, fish, red meat, and even dairy products
Crawford J, Dale DC, Lyman GH
Dihydrothymine is then converted to N -carbamoyl-BAIBA via the action of dihydropyrimidase (encoded by the DPYS gene)
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