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WADA, for better or worse, does not require substantive evidence to include substances on its banned list
The reactions were allowed to incubate in the dark at 37 C for 30 min, during which fluorescence was measured in a SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) using excitation in the range of 530560 nm and emission detection at 590 nm
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