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Forty-nine subjects were enrolled and tested before, during, and after fasting

Ravna AW et al (2009): Structure and localisation of drug binding sites on neurotransmitter transporters

Cell Lysis and Western Blot Cell pellets were lysed in cold radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris-HCl, 150 mM NaCl, 10 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid sodium salt and complete Protease and Phosphatase Inhibitor cocktails (Roche Molecular Biochemicals, Indianapolis, IN, USA) pH 7.4), and the amount of the extracted proteins was determined by Micro bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific, Waltham, MA, USA)