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Primary antibodies were diluted in TBST skimmed milk 5% buffer and the incubation was performed overnight at 4C using an orbital shaker followed by 2 h at room temperature with the secondary horseradish peroxidase (HRP)-linked antibody (Goat Anti-Mouse or Anti-Rabbit IgG Peroxidase Conjugated by Thermo Scientific)
At the same time, you feel a sense of fullness, so you feel less hungry
Saharan, S
Doxycyclin-inducible GIPR-overexpressing mice (TRE-GIPR mice) were generated in-house at The University of Texas Southwestern Medical Center as described previously 41
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