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A 20-minute exposure to this concentration resulted in all seizure-like behavioral phenotypes and demonstrated lower mortality compared to a 15 mM concentration in adult zebrafish (Mussulini et al., 2013), indicating that 10 mM is a suitable concentration for studying new potential antiseizure molecules (Canzian et al., 2019

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Chem Res Toxicol 1995, 8: 671-682

It is important to stress that insufficient levels of B12 raise susceptibility to infections and diseases, and are very harmful to the nervous system which in turn can cause neurological disorders and severe and sometimes irreversible nerve damage