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Maintaining low deuterium levels in mitochondrial water is an essential aspect of metabolism, and a failure in this regard leads to severe mitochondrial dysfunction and oxidative stress, associated with many chronic diseases ( Cytochrome P450 enzymes (CYPs), primarily localized to the endoplasmic reticulum or the mitochondria, oxidize a variety of different substrates, replacing a proton with a hydroxyl group in the substrate, splitting molecular oxygen, and producing water as the other product