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Key Takeaway Consistent supplementation, adequate Vitamin C intake and reducing lifestyle factors that increase oxidative stress may all help support healthy glutathione levels over the long term

Hamilos DL, Zelarney P, Mascali JJ

In vitro protein acyltransferase (PAT) assay Palmitoyl acyltransferase constituted by purified recombinant DHHC9 and GCP16 proteins (100 ng for each) were incubated with GLUT1 (2 g) in 25 l of reaction buffer (50 mM Tris-HCl [pH 7.4], 10 M palmitoyl alkyne-CoA, 1 M palmostatin B) at 25 C for 1 h followed by a Cu(I)-assisted click reaction with biotin picolyl azide (50 M) to biotinylate the proteins with the incorporation of palmitoyl alkyne