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Cells were cultured in Dulbeccos modified Eagles medium (DMEM) (Gibco, NY, USA) supplemented with 10 % foetal bovine serum (FBS) (Gibco, NY, USA) and were maintained in a humidified incubator at 37 C and 5 % CO 2

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The MT-II scaffold has since generated a large derivative family through substitutions at positions 6, 7 and 9, including N-methylated variants used to obtain MC1R selectivity

Circulation 130 , 15791588 (2014)