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Cell survival was determined using CCK8 assay (Solarbio, CA1210) according to the manufacturers instructions

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Glutamate neurotoxicity is independent of calpain I inhibition in primary cultures of cerebellar granule cells

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This finding should be viewed in the wider context of the existing premise that high plasma cystine is a marker of oxidative stress: studies have linked high cystine and low rGSH to multiple adverse outcomes, including CVD pathogenesis (Go and Jones 2011), renal disease progression (Rodrigues et al

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10.1507/endocrj.EJ12-0158 81

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