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A little science lesson
After 45 h of incubation with the secondary antibodies at room temperature, the pancreatic sections were subsequently stained with DAPI (1:500 in 1 PBS) for 30 min at room temperature, then rinsed and washed three times with 1 PBS and mounted
As described by Dr
To solve this issue, researchers developed a more chemically stable synthetic version known as Extendin, which they then combined with a mildly radioactive tracer used in standard PET scans
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