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To take into consideration the compositional nature of the data, all DESeq2-based results were complemented with graphical representations of abundance log-ratio (between finish and start matched samples) rankings

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Pellets were resuspended in 300 l PBS, and 10,000 events were analysed by flow cytometry using a BD Accuri C6 Plus flow cytometer (BD Biosciences) and FCS Express 7 Research (DeNovo Software)