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Health authorities discourage its use due to insufficient human safety data, and some jurisdictions have restricted it as a prescription-only medicine despite it not being available through legitimate prescriptions

Each patient will receive prescription medication to help combat the side effects should they arise along with directions on dosage to help keep side effects to a minimum

In general, single-end sequencing is sufficient in case of well-annotated organisms and frequently focuses only on mRNAs, while pair-end sequencing is recommended for de novo transcript discovery or isoform analysis