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The diluted genomic DNA was added to a 10 l reaction mix containing 0.2 mg/ml BSA, 4 mM DTT, 0.10% Tween, 0.1 mM dTTP, 1X phi29 buffer, and 15 U phi 29 DNA polymerase
For systemic or musculoskeletal targets, the peptide must absorb, survive first-pass metabolism or proteolysis in the portal circulation and liver, and reach the target tissue at an effective concentration
Phase D: Cortisol output may fall below the minimum threshold for normal function
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