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MOTS-c is administered subcutaneously 3 to 5 times per week

Hydration is supported throughout and the doctor or nurse checks in at regular intervals

MS2 analysis consisted of quadrupole isolation (isolation window 0.7) of precursor ion followed by collision-induced dissociation (CID) in the ion trap (AGC 1.8E4, normalized collision energy 35%, maximum injection time 120 ms)