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Liu Y, Tian Y, Tian Y, Wang Y, Yang W
2.15.2 Neural function defect score (NSS) Twenty-four hours post-infarction, neurological deficits were scored using the Masao Shimizu Sasamata method ( 2.16 Immunohistochemical analysis Mice were humanely sacrificed by deep anesthesia using an overdose of pentobarbital, followed by transcardial perfusion with phosphate-buffered saline (PBS) to clear the blood
Rat beta-NGF DuoSet ELISA, R&D Systems, Cat# AF-556-NA) were analysed in the posterior cortex by enzyme-bound immunosorbent assay (ELISA) in a microplate photometer using commercial kits (Peprotech, So Paulo, Brazil)
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