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Additionally, in order to explore the mechanism of action being neuronal apoptosis, the research team assessed levels of neuroinflammation and glial activation by detecting levels of IL-1-beta, IL-10, and TNF-alpha in the brain
Viability, early apoptosis, and mortality assessment Following the manufacturers protocol, early apoptosis was assessed using Annexin V staining (Invitrogen, Thermo Fisher Scientific, Barcelona, Spain)
& Huber, W
Kawakami, Y., Tanda, M., Adachi, S
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