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difficile (DSM 27543) was cultured in BHI medium supplemented with 5 g l 1 yeast extract, 0.1% (w/v) L-cysteine, 1 mg l 1 resazurine in an anaerobic (anaerobic: 5% H 2 , 10% CO 2 and 85% N 2 ) chamber (Whitley DG250 anaerobic working station)
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Protein samples (20 g) were separated on NuPage Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes
Journal of Psychiatry & Neuroscience: JPN, 36 (2), 78-86
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