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Gut-brain signaling via vagal pathways provides another important route for GLP-1's central nervous system effects

Immunofluorescence staining Primary murine chondrocytes and RAW 264.7 murine macrophage cell line were fixed with 3.7% PFA for 30 min at RT, permeabilized with TBS + 0.05% Tween 20 buffer for 30 min at RT, and blocked with 2.5% normal horse serum for 20 min at RT