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For measuring O 2 levels, the reaction system contained 50 mM phosphate buffer (pH 7.8) supplemented with 17 mM sulphanilic acid, 10 mM hydroxylamine hydrochloride, and 7 mM 1-naphthylamine, with absorbance determined at 530 nm (Liu et al., 2007)
A 2-ml sample volume was taken at regular intervals to maintain sink conditions and replaced with an equivalent volume of fresh buffer
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Additionally, glucose deprivation was observed to induce SLC7A11 expression in cancer cells
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