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Freezing creates ice crystals that can physically damage the peptide structure
Age groups and sample sizes were chosen based on prior experience with this model, feasibility of tissue and cell yields for each assay, and established practice for comparable omics and cytometry experiments in killifish
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Animal studies use controlled purity, defined dosing, and monitoring that home use cannot replicate, and they still do not answer questions about long-term human exposure, immunogenicity, or rare serious events
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