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The mRNA dots were quantified using the StarSearch tool developed by the Raj Lab ( DAPI was used to count the number of cells in each field

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Commonly used activators include HOBt (1-hydroxybenzotriazole), HBTU (2- (4-hydroxyphenyl) -4,5-dihydro-4-methyl-6-nitroimidazolo [1,2-a] pyridine-3-one), TBTU (2- (2-pyridyldithio) benzotriazole sodium salt), etc

PRPP is an intermediate in purine nucleotide synthesis, generated from ribose 5-phosphate from the pentose phosphate pathway 21