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The reduced complexity of the DNA sequences after bisulfite conversion (very few Cs and many Ts), together with high-homology regions of the human genome, especially in CG2 and the presence of several genotypes/quasispecies, makes it difficult to have a set of BS-specific primers to successfully amplify the HBV genome of interest in infected tissues

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His research has been supported for more than 35 years by grants from the National Institutes of Health

27 , 28 Understanding the implications of this pharmacologic variability in the context of STRIDE is, therefore, also essential