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Petcherski and Kimble, 2000)
Growth Hormone Regulation of MGF Expression In C2C12 mouse muscle cell lines, MGF mRNA increased within 1 hour of growth hormone stimulation preceding the increase in IGF-1Ea mRNA, which required 4 hours with a parallel increase in MyoD and M-cadherin expression, suggesting that GH directly and preferentially upregulates MGF expression prior to IGF-1Ea, potentially driving the satellite cell activation phase of muscle repair
10 L of isotonic saline containing 100 g/mL Nle-YI-(6) aminohexanoic amide as an internal standard is added to each sample on ice
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