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When selecting for removal of the plasmid in the presence of copper, we were able to recover multiple clones with gspD deleted from the chromosomal locus, with a success rate of ~25% (whereas ~75% were wildtype)
Plant J 77:367379 Neuefeind T, Huber R, Dasenbrock H, Prade L, Bieseler B (1997) Crystal structure of herbicide-detoxifying maize glutathione S-transferase-I in complex with lactoylglutathione: evidence for an induced-fit mechanism
Indeed it consists of two transmembrane domains, a large extracellular domain, and two short intracellular domains that are required for CD36 function after substrate binding (Su and Abumrad, 2009)
For example, a meta-analysis found that two copies of the MTHFR C677T variant (A/A, homozygous) increase the relative risk of heart disease by 38%.[ref][ref] However, not all studies agree, and a large prospective study (n=6,000) found that older people with two copies of the C677T allele were at a 30% decreased risk of death from cardiovascular disease when other parameters were included.[ref] The increased risk is not just due to high homocysteine
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