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RINm5F cells were treated with indicated chemicals for 17 hr, and then the cells were trypsinized and washed cells twice with cold BioLegends cell staining buffer, and then the cells were resuspended in Annexin V binding buffer at a concentration of 1 10 7 cells/ml
Of note, emerging multi-agonists, including the investigational triple agonist retatrutide, appear to share the class-related predominance of dose-dependent gastrointestinal adverse events
End of study sample collection At the end of the study, at experimental day 140, an aliquot of 25 g of excreta sample was collected from each cage and freeze-dried (James Hutton Institute, Aberdeen, UK)
