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Availability of data and materials The sequence read data of gut microbiome for characterizing TMAO producers (BioProject:PRJNA589723) were all available on NCBI sequence read archive (SRA) database (SRA accession numbers:SRR10465787-SRR10466048)

While traditional models often address hyperandrogenism and IR as primary, parallel drivers of PCOS pathology, they frequently fail to account for the rising evidence of systemic low-grade inflammation and immune dysregulation in the syndrome, particularly the pervasive role of the gut-metabolic axis [2, 16, 17]

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For cell culture experiments, sample sizes were not chosen on the basis of statistical methods