At 2 h after plating, the plating medium was replaced with fresh culture medium consisting of DMEM supplemented with 1 mM sodium pyruvate, 100 units/ml penicillin, 100 g/ml streptomycin, 0.2 % (vol/vol) of bovine serum albumin fraction V (instead of FBS), 100 nM dexamethasone, and 1 nM insulin (SigmaAldrich #I9278)
During these years, he spent much time hiking in the San Gabriel Mountains, and in regional national parks (Sequoia, Yosemite, Joshua Tree, and Grand Canyon), and was particularly fascinated by living things
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Myostatin regulates preadipocyte differentiation and lipid metabolism of adipocyte via ERK1/2