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RT-PCR ORSCs were treated with 0, 1, and 10 M morroniside, and total RNA was isolated using TRIzol reagent (Ambion, New York, NY, USA)

Factor TB-500 weekly exposure (typical) Pre-blended 1:1 vial 1.257.0 mg/week (steady, daily) Separate vials 4.010.0 mg/week (pulsed, 2x/week loading) Factor Reconstitution complexity Pre-blended 1:1 vial One vial Separate vials Two vials, two reconstitution steps Factor Best for Pre-blended 1:1 vial Simplicity, steady exposure Separate vials Injury-focused planning, dose tuning Both formats cover the same total compounds

It is possible that, in the context of RA, L-carnitine may impact TGF-1 levels indirectly or require longer treatment durations to manifest a measurable effect, given the complex regulatory networks involving TGF-1 in fibrosis and immune responses

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