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Excessive weight gain associated with sulfonylureas and insulin treatment is not benign and results in worsening of well-known cardiovascular risk factors, potentially contributing to the increased macro-vascular risk

An HPLC result estimates how much of a sample corresponds to the target compound

Another study of Raghunath et al

Chromatography was performed on a Kinetex 2.6 m HILIC 100 , 100 2.1 mm internal diameter column (Phenomenex), with a constant flow rate of 250 l/min and a solvent gradient from 20% Mobile Phase B (50:45:5, ACN:H 2 O:100 mM AmFm in H 2 O) to 22% B at 5 min, 40% B at 10 min, 60% B at 13 min, 80% B at 15 min, and 99% B at 20 min