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The cells were incubated at room temperature for 30 minutes in the dark, and the cell cycle was analyzed by flow cytometry (Win Bryte HS cytometer [Bio-Rad], using software Win Bryte, Bio-Rad Laboratories Inc., Hercules, CA, USA)
Mechanistically, by covalently attaching to the serine residue in the pancreatic lipase's active site, orlistat deactivates the enzyme
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To identify the distribution and cell localization of GLP-1R in the TNC, we performed immunofluorescence staining
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