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Uba1 was PCR-amplified from cDNA and cloned into pGEX-4T1 vector, which introduced an N-terminal GST tag followed by a TEV cleavage site and a C-terminal His tag
Okra contains insoluble fibre that helps clean the digestive tract by moving waste out of the body faster
25 Classic examples are -lactamases, which hydrolyze the -lactam ring of penicillins, cephalosporins, monobactams, and even carbapenems
9 Importantly, the key enzyme of GSH synthesis, gamma-glutamyl-cysteinyl-synthetase, is already active by the 2nd trimester of pregnancy, suggesting enzymatic immaturity is unlikely to be a primary limiting factor
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