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Use this workflow when: You are detecting HA, FLAG, His, Myc, GFP, or another tagged construct in transfected, transduced, or engineered samples

A paxillin-mCherry expression vector was built by PCR and cloning using a Gallus Gallus Paxillin-DsRed vector (a gift from Christopher Turner, Upstate Medical University) and pmCherry-N1 (Clontech) as a template for mCherry (Supplementary Table 1)

Change one variable at a time so you can attribute any changes in how you feel to the correct cause

Rapid weight loss, reduced appetite, and altered nutrient intake can negatively affect bone density a particular concern for women who already face hypoestrogenic states from certain endometriosis treatments