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and all diets were formulated to meet nutrient requirements of the National Research Council ( Cell Culture and Treatment IPEC-J2 cells were obtained from ATCC and cultured in cell culture flasks or plates (Nunc.) with DMEM-F12 (Gibco, Grand Island, NY, United States) supplementing 10% FBS, penicillin (100 U/mL), and streptomycin (100 g/mL), at 37C in a 5% CO 2 atmosphere humidified incubator

This is the phase that creates longer-lasting results, even after blood levels drop