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Cells were treated with Erastin (Selleck, S7242), IMP-366 (AOBIOUS, AOB6657), DFO (Sigma, D9533), Fer-1 (Selleck, S7243), Lip-1 (Selleck, S7699), iFSP1 (Cayman Chemical, 29483), Z-VAD-FMK (Selleck, S7023), MCC950 (Selleck, S8930), Nec-1 (Selleck, S8037), and IVM (APExBIO, A2813)
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Homologous recombination events were confirmed by Southern blot analysis, and positive clones were injected into blastocysts derived from C57Bl/6 mice