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After centrifuged and washed with PBS three times, the cell pellet was resuspended and cultured in Dulbeccos Modified Eagles Medium (DMEM) containing 10% FBS and cultured at 37 C in a humidified atmosphere with 5% CO 2

And if they are not yet diabetic, losing weight would be the best way to prevent progression of the condition to diabetes

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Unbelievably, a little over a year later, usage is up to 10% (10 years ahead of schedule) with no signs of slowing