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Using a human intestinal epithelial platform with functional L-cells, researchers can now directly stimulate the epithelium and measure endogenous GLP-1 and other incretin secretion in a physiologically relevant context enabling mechanistic interrogation of secretion biology upstream of receptors

The ABHD5 gene is located on chromosome 3p21.33 and is composed of 10 exons that generate four alternatively spliced mRNAs that collectively encode three distinct protein isoforms

Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26

This causes cellular damage and increases oxidative reactions