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Glutathione is often referred to as the master antioxidant
Therapeutic siRNA: state of the art
For each assay, cells were plated in duplicate, with one plate containing CellTox Green Dye added according to the Express, No-Step Addition at Seeding protocol described in the CellTox Green Cytotoxicity Assay Technical Manual #TM375 (10l of CellTox Green Dye per 5ml of cells)
The resultant files were processed with El-Maven (Elucidata) alongside the Kyoto Encyclopedia of Genes and Genomes (KEGG) 20,21,22 database for metabolite assignment and manual peak integration as previously described using intact mass, isotopic patterns, and an in-house standard library 23
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