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The slides were developed with DAB until the signal clearly appeared, and the nuclei were stained with hematoxylin for 5 min at room temperature, and images were obtained using a BX53 microscope (Olympus Corporation)
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At the systemic level, GLP-1 RAs improve insulin sensitivity in peripheral tissues (muscle, adipose), reduce circulating free fatty acid flux to the liver, suppress hypothalamic appetite circuits to promote weight loss, and lower systemic pro-inflammatory cytokines (TNF-, IL-1, IL-6), thereby reducing the hepatic inflammatory milieu indirectly [7,26,30]
However, there is a need to standardize parameter measurements (such as TEWL, collagen density, viscoelasticity, etc.) in randomized, placebo-controlled trials in designs that include estrogen-deficient skin in menopausal women with correlations to biomarkers examined in these clinical studies that assist patients and physicians as to how to evaluate, select, and provide the most effective treatments in the future
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