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While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain
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HPLC analysis and FTIR spectroscopy to confirm the structural integrity and purity of the final product
Serious life-threatening allergic reactions including death have happened in people who have received vitamin B12 by injection or by intravenous (IV) infusion (parenteral)
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