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Induction of HO-1 reduced inflammatory cytokines, such as TNF- and IL-6, increased antioxidants, and improved angiogenesis in wound tissues of diabetic rats, thereby wound healing was accelerated in the diabetic state [118]
Three colonies from the resulting plates were picked for each and grown overnight in 1 ml of M9-glycerol medium containing kanamycin in a 2 ml 96-deep-well plate (Corning, P-DW-20-C-S)
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