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The needle should be inserted at a slight angle through the septum center, and an equivalent volume of air should be injected into the vial before withdrawing liquid to prevent vacuum formation
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Chemical structure of melatonin The molecular structure of melatonin is described as N-acetyl-5-methoxytryptamine, indicating a methoxy group at the 5-position of the indole ring and an acetylated amine group at the terminal end, with a bicyclic indole ring system fused to a six-membered benzene ring, an acetamide side chain, and a methoxy group as key substituents [27]
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