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Weighed cellular pellets were solubilized in ice-cold RIPA buffer (150 mM NaCl, 1% Nonidet NP-40, 1% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0) supplemented with a phosphatase inhibitor cocktail (1:200, Sigma-Aldrich P5726) and Benzonase nuclease (1:1000, Merck 70,664)
Yes, when prescribed and monitored by a qualified provider at therapeutic doses
This can be addressed by selectively targeting specific PKA subunits
doi: 10.3892/br.2020.1372
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