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Some of the factors that have the most significant influence on weight changes during menopause include: Reduced estrogen levels Menopause coincides with a decline in estrogen levels, which can affect the distribution of fat and lead to increased fat storage around the waist

Sources: Fras, Juan P., et al

At ~50% confluency, cells were co-transfected with full-length Flag-tagged or MYC-tagged substrate constructs and HA-tagged protease constructs using Lipofectamine 3000