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Sample homogenate proteins (2,5 and 5 g/lane to SOD-1 and catalase, respectively) were separated by SDS-PAGE electrophoresis (15% polyacrylamide) according to literature-based methods and electrophoretically transferred to a nitrocellulose membrane by electroblotting (150 mA V, 2 h)
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The reference range for B12 can vary from lab to lab but it is usually between 200 700 pmol/l or pg/ml if you are in the U.S.A
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