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On the contrary, some HSV-1-induced H2AX foci were still detected in neurons infected in the presence of PAA (Figures 2B,d,h) that blocks viral DNA synthesis, but not transcription of early genes, suggesting that viral DNA replication is not essential but may partly contribute to the accumulation of H2AX
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On the other hand, oxidative stress leads to an increase in the release of cytochrome C from the mitochondria which activates the apoptotic mechanism in the cells by activating caspases (caspase-3/7, 8 and 9) and pushing the cells to the programmed cell death
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